Interactions of FliJ with the Salmonella Type III Flagellar Export Apparatus
نویسندگان
چکیده
منابع مشابه
Interaction between FliJ and FlhA, components of the bacterial flagellar type III export apparatus.
A soluble protein, FliJ, along with a membrane protein, FlhA, plays a role in the energy coupling mechanism for bacterial flagellar protein export. The water-soluble FliH(X)-FliI(6) ATPase ring complex allows FliJ to efficiently interact with FlhA. However, the FlhA binding site of FliJ remains unknown. Here, we carried out genetic analysis of a region formed by well-conserved residues-Gln38, L...
متن کاملInteraction between FliJ and FlhA , components of the 1 bacterial flagellar type III export apparatus
1 Interaction between FliJ and FlhA, components of the 1 bacterial flagellar type III export apparatus 2 3 Tatsuya Ibuki, Yumiko Uchida, Yusuke Hironaka, Keiichi 4 Namba, Katsumi Imada, and Tohru Minamino 5 6 a Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan 7 b Department of Macromolecular Science, Graduate School of Science, Osaka 8 University, 1-1 Machikaneyama...
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We isolated and characterized spontaneous mutants with defects in the 147-amino-acid Salmonella protein FliJ, which is a cytoplasmic component of the type III flagellar export apparatus. These mutants, including ones with null mutations, have the ability to form swarms on motility agar plates after prolonged incubation at 30 degrees C; i.e., they display a leaky motile phenotype. One mutant, SJ...
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Flagella are assembled sequentially from the inside-out with morphogenetic checkpoints that enforce the temporal order of subunit addition. Here we show that flagellar basal bodies fail to proceed to hook assembly at high frequency in the absence of the monotopic protein SwrB of Bacillus subtilis. Genetic suppressor analysis indicates that SwrB activates the flagellar type III secretion export ...
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The bacterial flagellum contains its own type III secretion apparatus that coordinates protein export with assembly at the distal end. While many interactions among export apparatus proteins have been reported, few have been examined with respect to the differential affinities and dynamic relationships that must govern the mechanism of export. FlhB, an integral membrane protein, plays critical ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2003
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.185.18.5546-5554.2003